maldi tof microflex lrf mass spectrometer  (Microflex Inc)

Journal: International Journal of Molecular Sciences

Article Title: Cystatin SN (CST1) as a Novel Salivary Biomarker of Periodontitis

doi: 10.3390/ijms241813834

Figure Lengend Snippet: Two-Dimensional Gel Electrophoresis of saliva sol phase samples. Unambiguously identified spots by MALDI-TOF-MS are circled in red and numbered. Spot number 3 is circled in blue, indicating the alleged position of Cystatin SN (CST1) in P samples.

Article Snippet: Finally, 0.8 µL aliquots of this mixture were released on the metal target plate of a Microflex ® LRF MALDI-TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) in reflector mode.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: Schematic illustration of the MALDI-TOF MS profiling of plasma exosomes for the evaluation of osteosarcoma lung metastasis

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques:

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: Characterization of exosomes isolated from 143B osteosarcoma cell culture medium (A–D) (A) TEM micrographs, (B) Western blot analysis of exosome markers, (C) NTA measurement (1000-times dilution), and (D) MALDI-TOF MS fingerprints in the range m/z 2000–15,000. Scale bar: 200 nm.

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques: Isolation, Cell Culture, Western Blot

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: MALDI-TOF mass spectra of exosomes derived from different cell lines and PCA and hierarchical clustering of the MALDI mass spectra (A) MALDI-TOF mass spectra of exosomes from 3T3E1, 143B, HOS, MG63, and U2OS cells. Main peaks of exosomes were labeled in the figures. (B–D) (B) PCA of the MALDI-TOF mass spectra obtained from 3T3E1, 143B, HOS, MG63, and U2OS exosomes. Hierarchical clustering results of 3T3E1, 143B, HOS, MG63, and U2OS cell lines based on the MALDI-TOF mass spectra of (C) exosomes or (D) cells, shown as heatmap using Euclidean distance measure and Ward linkage. For MALDI-TOF analyses, six replicates were performed on each cell line.

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques: Derivative Assay, Labeling

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: MALDI-TOF mass spectra of plasma-derived exosomes for the identification of osteosarcoma (A) MALDI-TOF mass spectra of plasma-derived exosomes isolated from a patient with osteosarcoma and a healthy volunteer. (B) PCA of the MALDI-TOF data of plasma-derived exosomes isolated from 12 healthy controls (H) and 12 patients with osteosarcoma (O). (C) Important MALDI-TOF MS features of plasma-derived exosomes from the 12 patients with osteosarcoma (O) compared with the 12 healthy volunteers (H) identified by PLS-DA. For MALDI-TOF analyses, three replicates were performed on each sample.

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques: Derivative Assay, Isolation

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: MALDI-TOF mass spectra of plasma-derived exosomes for the identification of osteosarcoma lung metastasis (A) MALDI-TOF mass spectra of plasma-derived exosomes from a patient with osteosarcoma lung metastasis and a patient with non-lung metastasis. (B) PCA of the MALDI-TOF data of plasma-derived exosomes from 10 patients with osteosarcoma lung metastasis (L) and 10 patients with non-lung metastasis (N). (C) Important MALDI-TOF MS features of plasma-derived exosomes from the 10 patients with osteosarcoma lung metastasis (L) compared with the 10 patients with non-lung metastasis (N) identified by PLS-DA. (D) ROC curve based on 7 significant features ( m/z = 3266, 3730, 5104, 5135, 5272, 5344, and 7626) plotted by 100-times Monte Carlo cross-validation (MCCV) using MetaboAnalyst 4.0. For MALDI-TOF analyses, three replicates were performed on each sample.

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques: Derivative Assay

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: Seven protein biomarkers of osteosarcoma lung metastasis identified by MALDI-TOF and LC-MS/MS

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques: Molecular Weight

Journal: iScience

Article Title: Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

doi: 10.1016/j.isci.2021.102906

Figure Lengend Snippet: Validation and gene ontology analysis of the 7 protein biomarkers for osteosarcoma lung metastasis (A and B) Validation of the 7 protein biomarkers using the MALDI-TOF mass fingerprints of plasma-derived exosomes from another (A) 10 patients with non-lung metastasis osteosarcoma (N) and (B) 10 patients with lung metastasis osteosarcoma (L). The PCA model was built with the training data set shown in Figure 4 B. For the test data set, two MALDI-TOF replicates were performed on each sample. (C) Gene ontology enrichment of the 7 protein biomarkers. BP: biological processes; CC: cell component; MF: molecular function.

Article Snippet: MALDI-TOF analysis was performed on a Microflex LRF MALDI-TOF mass spectrometer (Bruker, Bremen, Germany) equipped with a pulsed 337-nm nitrogen laser using SA (20 mg/mL in 50% acetonitrile [ACN]/50% water/0.1% TFA v/v/v), HCCA (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v), and DHB (10 mg/mL in 30% ACN/70% water/0.1% TFA v/v/v) matrix.

Techniques: Derivative Assay